Counterimmunoelectrophoresis in the serodiagnosis of Legionnaires' disease.
نویسنده
چکیده
Counterimmunoelectrophoresis (CIE) was found to be a rapid, specific method for detecting circulating antibodies to Legionella pneumophila, using a simple ultrasonically disrupted antigen. The diagnostic specificity of the technique was 93% and the diagnostic sensitivity was 86.3%. Both IgM and IgG classes of antibody can be detected. There is some cross-reactivity between serogroup 1 and serogroup 6 but none between serogroup 1 and serogroups 3, 4, and 5. It is suggested that CIE may provide a simple, reproducible screening test for Legionnaires' disease.
منابع مشابه
The diagnosis of Legionnaires ' disease by counterimmunoelectrophoresis MALCOLM
Counterimmunoelectrophoresis (CIE) was found to be a rapid, specific method for detecting circulating antibodies to Legionella pneumophila, the aetiologic agent of Legionnaires' disease in human sera. Optimum conditions for performing the test are given. Better precipitin lines are observed when the gel support is neutral, and a result may be obtained in 90 to 180 minutes. Comparison of results...
متن کاملThe diagnosis of Legionnaires' disease by counterimmunoelectrophoresis.
Counterimmunoelectrophoresis (CIE) was found to be a rapid, specific method for detecting circulating antibodies to Legionella pneumophila, the aetiologic agent of Legionnaires' disease in human sera. Optimum conditions for performing the test are given. Better precipitin lines are observed when the gel support is neutral, and a result may be obtained in 90 to 180 minutes. Comparison of results...
متن کاملSerology of Legionnaires disease: comparison of indirect fluorescent antibody, immune adherence hemagglutination, and indirect hemagglutination tests.
An immune adherence hemagglutination (IAHA) test for the measurement of antibodies to Legionella pneumophila was developed and evaluated for the diagnosis of Legionnaires disease. Its sensitivity was compared to that of the indirect fluorescent antibody (IFA) test and a recently developed indirect hemagglutination (IHA) test. The sensitivity of the three tests appeared to be similar, with the I...
متن کاملSimultaneous Detection of IgA/IgG AntiTissue Transglutaminase/Deamidated Gliadin Peptides in Serodiagnosis of Celiac Disease
Background: Celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. However, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. The seronegative patients with celiac disease can i...
متن کاملA Case Report of Severe Legionnaires’ Disease in Tehran, Iran
Legionella pneumophila is a thin, aerobic, Gram-negative, pathogenic bacillus and the leading cause of Legionnaires’ disease (LD) in human, especially those with high risk conditions including immune system disorders or chronic lung diseases. The mortality rate of LD has been reported to be 60% or higher in inappropriately treated cases. Investigating the prevalence of L. pneumophila and identi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 36 4 شماره
صفحات -
تاریخ انتشار 1983